DNA Extraction and Purification
Biochain Institute Inc FFPE Tissue DNA Extraction Kit, 1 kit/PK
BioChain's FFPE isolation kit is a unique product, with unique benefits and applications. It is not a true purification kit. Instead, it quantitatively releases both DNA and RNA from FFPE tissues, without extensive downstream purification. This kit uses a non-volatile, non-toxic reagent to de-paraffinize, and then employs enzymes to digest FFPE tissue. The tissue digests are then subjected to de-crosslinking of the DNA and RNA released from the tissue. When these tissue digests are diluted 1:2, they form a good template for PCR, qPCR, qRT-PCR and similar applications. Thus, this kit provides a simple way to release nucleic acids from FFPE tissue without risking the yield losses that can result from purification steps. The kit contains sufficient reagents for 100 FFPE tissue DNA extraction reactions.
Research Products International Corp Quick-DNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 50 Preps
The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
Research Products International Corp ZymoPURE Plasmid Miniprep Kit, 100 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
Biochain Institute Inc Genomic DNA - Lupus: Spleen, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Skeletal Muscle, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (CALD1/1424R), Biotin conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
Research Products International Corp ZR-96 DNA Clean & Concentrator-5 Kit, 4 x 96 Preps
The ZR-96 DNA Clean & Concentrator-5 products provide purification of up to 5 µg DNA per well from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. The product facilitates the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Clean and concentrate up to 5 μg DNA (per well) with ≥ 10 μl elution volume in as little as 15 minutes with 0 μl wash residue carryover.
Deep-well filtration plate designs allow DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Biochain Institute Inc Genomic DNA - hypertension: Artery, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - hypertension: Kidney, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Epoch Life Science Inc GenCatch™ Blood &Tissue Genomic Miniprep Kit (50 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast, cost-effective method to extract gDNA ready for downstream use from various starting materials (such as bacteria, yeast, blood, cells, tissue, feces, etc.). Silica membrane technology. 120 ug Binding capacity. Instructions for sample type listed in protocol. Used by labs around the world. 18 month shelf life.
Biochain Institute Inc Control Genomic DNA - Bovine Male, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Bioworld Blood Genomic DNA Isolation (Micro) Kit, 50 Prep(s)
For the rapid preparation of high quality DNA from whole blood, buffy coat, plasma and serum Input volumes up to 150 µL No phenol required for DNA isolation Extract DNA from whole blood, buffy coat, plasma and serum Fast and convenient spin column protocol FEATURES AND BENEFITSNo Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals Fast and easy processing -Rapid spin-column format allows for the processing of multiple samples in 30 minutes. Isolate genomic DNA from various inputs - Isolate DNA from various blood inputs including plasma, whole blood, buffy coat and serum Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis. High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Micro Kit is observed.
LGC GENOMICS LLC (Lucigen) sbeadex™ maxi Plant DNA Purification Kit (80-100 mg starting material), 960 purifications
Highly efficient, magnetic bead based purification of DNA from plant samples.